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Simultaneously, the CMS Interoperability and Prior Authorization Final Rule (CMS-0057-F) became operational on January 1, 2026, requiring Medicare Advantage, Medicaid, and CHIP plans to streamline PA with shorter decision timelines (72 hours for expedited, 7 days for standard) and begin publicly reporting PA metrics ( [16] )

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Isopropyl -d-1-thiogalactopyranoside (IPTG) was added to a final concentration of 1 mM and the culture was further incubated at 18 C overnight