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Then estimation was made on the basis of reduction of GSSG in the presence of NADPH and glutathione reductase (GRx), and decrease of NADPH at 340 nm after initiating the reaction by adding GRx was taken as an index of GSSG content, which was evaluated and expressed as mol/gHb (14)

First, reactive Cys326 of FBXO22 is suggested to form a covalent hemithioacetal with the UNC10088 aldehyde derivative and is therefore expected to be critical for complex formation