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Samples were then centrifuged at 200 g at room temperature for 5 min, the supernatant was discarded, and the cells were resuspended in PBS

2016;18:23 LaRocca TJ, Stivison EA, Mal-Sarkar T, Hooven TA, Hod EA, Spitalnik SL, et al

Denaturing buffers use harsh reagents such as urea, guanidine HCl, and SDS to denature the structure of Protein A, G, or L and the antibodies to disrupt the interactions

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The authors also validated that GSH content and GPX4 activity were significantly decreased when H22 cells were co-incubated with Mn-MOF (Figures 5H, I)

Exome-wide rare variant analysis identifies TUBA4A mutations associated with familial ALS