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The purified non-acylated tirzepatideGLP-1Rmini-G s Nb35 complex (3.5 L) was applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3, 300 mesh), and subsequently vitrified using a Vitrobot Mark IV (ThermoFisher Scientific) set at 100% humidity and 4 C

Liposomal delivery systems have been shown to promote the retention of collagen within artificial membranes and elevate the expression of structural skin proteins such as collagen, keratin, and involucrin, contributing to improved skin elasticity and reduced signs of ageing [212]

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