While cytosol, peroxisomes, mitochondria, and plastids in Arabidopsis wild-type plants maintain a highly reduced glutathione buffer with redox potentials of less than 310 mV, the ER is far less reducing with E GSH values of less than 240 mV (Meyer et al., E GSH with high specificity due to selective mediation of thiol/disulfide exchange reaction between glutathione and roGFP by GRXs (Meyer et al., E GSH is outside the measuring range of these probes thus limiting their usability
le Roux CW, Welbourn R, Werling M, et al
Annexin V/PI staining was used to detect apoptotic and necrotic cells, where Annexin V +/ PI cells are considered the early apoptotic and Annexin V +/PI + cells are considered necrotic or late apoptotic
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