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First we assessed proteolytically active proteasome subunits MECL1 , LMP2 and LMP7 required for assembly of the 20 S proteasome, protein degradation and amino acid recycling 25 , finding IGF-induced MECL1 downregulation in DU145 cells only and LMP2 downregulation in both DU145 and 22RV1 (Supplementary Figure S2A)