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After washes in PBS with 0.1% Tween (PBS-T) for 30 min (10 min per wash), a secondary antibody was applied for 1 h at room temperature in the blocking buffer (see Supplementary Table 4 for primary and secondary antibodies used in this study)

A French nationwide case-time-control study of completed suicides and suicide attempts found no specific risk related to prior psychiatric history or obesity during GLP-1 RA use ( 5 State of the art and future directions No clear causal mechanism linking GLP-1 RA use to suicidality has been established