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Protein bands were visualized using Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore, Billerica, MA, USA) and a ChemiDoc MP Imaging System with Image Lab Touch Software Version 1.0 (Bio-Rad Laboratories, Hercules, CA, USA)

Ma L, Fu Q, Xu B, Zhou H, Gao J, Shao X, Xiong J, Gu Q, Wen S, Li F, et al

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For the immunostaining of cultured PSCs, cells were fixed in 4% PFA for 15 min followed by a 15-min incubation in 0.5% Triton-X100

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Health 5 , e578e592 (2017)