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Total RNA was extracted from the cells of each treatment group by the TRIzol method, and the cDNA was synthesized by reverse transcription using total RNA as a template with Reverse Transcription Kit (Takara Bio Inc, Japan) at 37C for 15 min, 85 C for 5 s, and 4 C for 10 min

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The molecule is small enough to cross the epidermal barrier to some degree, but reaching fibroblasts in the mid-to-deep dermis where the structural work happens requires either injection or a delivery mechanism that bypasses the barrier

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