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Cells were washed to remove viral particles and transduced cells were selected in the presence of 1 g/ml puromycin (Sigma-Aldrich, P8833) for at least 96 h

In some embodiments, the otic formulation or composition comprises about 0.04% by weight of the cholesterol

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At lower weights such as 2 mg, the electrode surface may not have sufficient active sites, resulting in lower sensitivity and weaker electrochemical signals 48,49

The intestinal mucosa naturally produces mucus as a protective barrier, and changes in transit time might make this mucus more apparent in stool

Cell 7 , 867877 (2001)