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Two days after plating, various concentrations of glucose, GLP-1, and 007-AM in the modified Krebs-Ringer bicarbonate HEPES buffer (KRBH: 129-mM NaCl, 5-mM NaHCO 3 , 4.8-mM KCl, 1.2-mM KH 2 PO 4 , 1.2-mM MgSO 4 , 10-mM HEPES, 1-mM CaCl 2 ) containing 0.1% (v/v) BSA and 0.1-mM glucose were administered to the cells, which were then incubated for 1 h

On average, individuals may lose between 5-10% of their initial body weight in this time frame