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For negative controls, primers were directed at the m6A-negative region of SLC7A5, and for positive controls, they were directed at the m6A-positive region [23]

This finding reveals the metabolic flexibility of Tregs in the TME, reducing glucose dependence and enhancing lactate metabolism to maintain immunosuppressive function

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Additional evaluations, including the JC-1 assay, DCFH-DA staining, and mtDNA analysis, were performed to assess its effects [158]

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However, ongoing research into peptide therapies may eventually lead to their broader acceptance in orthopedic and sports medicine applications