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Human GST (A1, M1, M2, or P1) in 100 mM phosphate buffer (pH 7.4, 1.83.6 nmol protein amount) was added to react with produced NAPQI for 3 h

It is also reasonable to employ supportive measures as dictated by the patients clinical status

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Additionally, the use of a fixed 300 kcal mixed meal for all patients may have been suboptimal for older or larger adolescents, potentially contributing to the attenuated incretin responses observed over time