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The ROI was positioned so that one edge was placed at the periphery of the epidermis ensuring that the ROI extended over the epidermis and well into the dermis

We next treated each of the 1224 detected lipid molecules as a feature and used the EdgeR package 30 to identify those that showed statistically significant differences between each of the sterile mutants compared to WT worms

doi: 10.2337/dc11-0291

Most genetic diseases associated with PDHc deficiency are due to mutations in either of the two subunits ( encoded by the PDHA1 gene or encoded by the PDHB gene) of the functional PDH heterotetramer of the PDHc