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The primer sequences used and the sizes of caspase-3 and glial fibrillary acidic protein (GFAP) housekeeping -actin are shown in Table 1

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The eluate was further purified using SEC (Superose 6 increase 100/30 Cytiva) with the SEC buffer (20 mM Hepes pH7.5 and 400 mM NaCl)