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Then the cells were centrifuged and washed once with 1 ml of 0.1 M borax (Sigma-Aldrich, 71997), followed by another wash with 1% BSA in PBS

This is particularly important during periods of increased energy demand, such as exercise or fasting

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4 Some recommend the use of a loading dose before initiation of a maintenance dosing regimen to more quickly achieve therapeutic plasma concentration

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