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Future studies should prioritize large-scale, single-strain multicenter RCTs to validate long-term efficacy and safety, standardize detection protocols and experimental designs to reduce heterogeneity, and deepen mechanistic exploration of Clostridium /metabolitetarget cell interactions

for daily injections, rotate within one area for a week before moving to another site

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Even so, consumers are put in an awkward place

Recruitment occurred from August 2024 to March 2025 through local media and social media platforms