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Crystallographic refinement and atomic models of a human Fc fragment and its complex with fragment B of protein A from Staphylococcus aureus at 2.9- and 2.8-A resolution

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(1990a)

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Protein samples were separated by SDSPAGE, transferred onto PVDF membranes, and probed with primary antibody at 4 C overnight, followed by HRP-conjugated secondary antibody at room temperature for 1 h

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