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Precise control of operational parameterssuch as power, temperature, and extraction durationis essential, as excessive energy input or prolonged treatment can degrade sensitive polyphenolic structures, potentially lowering yield or altering compound profiles ( 3.1.4 Supercritical fluid extraction method Supercritical fluid extraction is an alternative two-step technique

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Antibiotic selection (puromycin 1.5 g/ml, Blasticidin 5 g/ml, or both) was started 36 h post-transduction

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IV glutathione, when medically supervised, generally shows faster and more consistent results due to higher bioavailability