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Genes were amplified using oligonucleotides (Table S1) and PowerUp SYBR green Master Mix following the manufacturers protocol (Applied Biosystem)

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Cells were mixed with 2 g of GFP-LC3B (Beyotime Biotechnology) and 100 nM Mito-Tracker Red CMXRos, then treated with the indicated concentrations of PTX to detect mitochondrial and autophagosome co-localization