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For example, some endogenous proteins in the matrix share similar hydrophobicity with bovine insulin, potentially interfering with analysis during the pretreatment process

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CYP2E1, Cytochrome P450 2E1

57 Furthermore, a subsequent prespecified analysis of the trial examined the effects of semaglutide in patients with and without history of HF at enrolment, with a significant reduction in MACE in patients with HFrEF and HFpEF and a 21% and 25% reduction in the HF composite endpoint, respectively

Step 2: Add 100ul HRP-Streptavidin (orange) into each well, seal the plate and static incubate for 30 minutes at 37C