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NICE guidance on NSAID use emphasises using the lowest effective dose for the shortest duration necessary, particularly in patients with gastrointestinal symptoms or risk factors

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The amplification reaction was performed in an ABI PRISM 7500 fluorescence quantitative PCR instrument using the following conditions: 50C for 2 min, 95C for 10 min and 95C for 15 sec, and a final extension step of 1 min at 66C, 40 cycles, stored at 4C

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