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Human monocytic cell line (THP-1) was purchased from ATCC (Manassas, VA) and maintained in RPMI-1640 supplemented with heat inactivated 10% FBS (Gemini Bio-Products, West Sacramento, CA)

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One possible explanation provided by gene expression data in 2024 suggests that smoking is significantly associated with the upregulation of the cytokine receptor-like factor-1 (CRLF1) gene, which translates to actin alpha 2 (ACTA2+) smooth muscle cells, particularly found in carotid plaques