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(1) Skp2 not only promotes the degradation of Myc but also enhances the transcription of a subset of Myc target genes with or without ubiquitin ligase activity 14,15,19

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Next, we performed real-time RT-PCR to determine the gene expression of osteoblast differentiation markers runt-related transcription factor 2 (RUNX2), osteocalcin (OCN), type I collagen (Col I), osterix (OSX), bone sialoprotein (BSP), and osteopontin (OPN) using RNA isolated from these cells