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The tobacco actin gene was adopted as the reference gene, and the expression levels of crucial genes such as OLP , SCR2 , CAT1 , PER1 , HIR1 , WRKY53 and PHO1 were determined using qRT-PCR

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Current smokers reported these symptoms at higher rates than ex-smokers, with ex-smokers experiencing urge-to-smoke (24.92%), restlessness (17.60%), and anxiety (15.41%)