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Abcam, Cambridge, UK) at 4 C overnight, and then were incubated with Alexa Fluor 594-conjugated Goat Anti-Mouse IgG(H + L)(SA00006-3, Proteintech, IL, USA) or Alexa Fluor 594-conjugated Goat Anti-Rabbit IgG(H + L)(SA00006-4, Proteintech, IL, USA)