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The cell line was cultured in basal bronchial epithelial cell basal medium (BEBM) (Lonza, Walkersville, MD) supplemented with 2 ml of bovine pituitary extract and 0.5 ml of: insulin, hydrocortisone, retinoic acid, transferrin, triiodothyronine, epinephrine, and human recombinant epidermal growth factor (Lonza, Walkersville, MD)

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The findings showed that cigarette smoke had a significant effect on the bacterial profiles in all sample types compared to the control group, and in the fecal and buccal swabs in comparison to the e-cigarette group

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