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Vacuum concentrated and dried fractions of saliva samples were reconstituted in 60 L of HPLC diluent (95% water, 5% acetonitrile, 0.1% formic acid) followed by sonication for 5 min and centrifugation at 16,000 g for 8 min

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mTOR is a member of the PI3K family and an effector protein downstream of the PI3K/AKT signaling pathway [125]

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