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Gene expression and pathway analysis for chronic smoke exposure To analyse changes in gene expression, passage 1 HBECs derived from 3 different donors were plated on 12 mm transwell-clear inserts and cultured for 1 month under air-liquid interface conditions (i.e

Conditioned media were probed for cytokine secretion by the V-PLEX Human Proinflammatory Panel II (4-Plex) (Mesoscale Discovery, K15053D-1) and growth factor secretion by the U-PLEX Development Pack (Mesoscale Discovery, K15228N-1) according to the manufacturers protocols