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Immunoprecipitation PMA-differentiated U937 cells were collected and lysed by RIPA buffer

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Many people say that when temperatures are low, especially below zero, they cannot smoke

IndJ Multidis Dent 2012

After blocking with 5% Bovine serum albumin (BSA) for 30 min, the slides were incubated with the primary antibodies against the following proteins at 4C overnight: smooth muscle actin (-SMA) 1:500 (Ab5694